The MOUSE ANTI HUMAN CD29 Antibody from MyBioSource.com is a Mouse Monoclonal antibody to CD29, GPIIA', integrin beta 1, integrin, beta 1a, and ITGB1. This antibody recognizes Human, and Other Mammalian antigen. The MOUSE ANTI HUMAN CD29 Antibody has been validated for the following applications: ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Western Blot.
Description
Mouse anti Human CD29 monoclonal antibody, clone 12G10 recognizes human CD29 also known as beta1 integrin or VLA-4 subunit alpha. CD29 is a ~130 kDa under reducing, ~115 kDa under non-reducing conditions single pass type I transmembrane glycoprotein. CD29 acts as the common beta subunit of the heterodimeric very late antigens 1-6, complexing with CD49a-f respectively where it forms part of the receptors for laminin, collagen and fibronectin. the VLA heterodimers ediate cell-cell and cell-matrix interactions. Mouse anti human CD29, clone 12G10 binding to cells adhering via VLA-4 results in actin cytoskeletal disruption and subsequent inhibition of attachment and spreading whilst 12G10 binding to cells adhering via VLA-5 results in enhancement of both these processes (Humphries et al. 2005). Clone 12G10 enhances alpha 5 beta 1-fibronectin interactions and binds to a region of CD25 containing the binding epitopes of several other anti CD29 antibody clones. However, unlike these, binding of 12G10 is enhanced in the presence of ligands such as fibronectin fragments (Mould et al. 1995). Binding of antibody clone 12G10 to the integrin beta1 subunit is affected by divalent cations and the binding epitiope appears to be located around residues 207-218 in the b1 subunit putative A-domain (Mould et al. 1998)